ELISpot Flex: Porcine TNF-α (ALP)

ELISpot Flex: Porcine TNF-α (ALP)

3135-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT32E9
Detection mAbMT859, biotin
Enzyme conjugateStreptavidin-ALP

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Delivery 4-9 business days

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ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting porcine TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Porcine TNF-α (ALP)
ApplicationELISpot
AnalyteTNF-α
ReactivityPig
SpecificityThis kit is based on a matched pair of mAbs specific for porcine (pig) TNF-α.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
PBMC from pig (20,000 cells/well) were incubated for 20 h in the absence or presence of LPS and the number of cells secreting TNF-α was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

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Tutorial, Published November 7, 2022

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Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
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ELISpot Plus: Porcine TNF-α (ALP)